Table 3.

Primers used in this study. Primer sequences specific for amplification of the hygB cassette are in bold.

Primer ID	Nucleotide Sequence 5′→3′
IVT-cycUP-fwd	TAATACGACTCACTATAGATTAGCCCTACACGTC
IVT-cycUP-rev	TTCTAGCTCTAAAACGGTTGACGTGTAGGGCTAA
IVT-cycDW-fwd	TAATACGACTCACTATAGGTTAGTTGGGATTCGC
IVT-cycDW-rev	TTCTAGCTCTAAAACAGCAGCGAATCCCAACTAA
5harm_cyc_Hyg_F	CTCACCAGGCTGTGGCAAGCAGTTGGCGTGTATATTAGCCCTACACGTCAACAGTTTAGCTTGCCTCGTC
3harm_cyc_HygB_R	TACTTCATATATCCAACCAAACAAAACACATACCCTAGTTTCTCACCCAGTCCAGTATAGCGACCAGCATT
cyc_out_F	CATTGTGCTGGACTTTGGGC
cyc_out_R	CCGGCTTTACTTTCATGGCG