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. 2021 Dec 9;26(24):7466. doi: 10.3390/molecules26247466

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of SCH on the Nerve fiber regeneration in NPCs in vitro. (a–j) neuronal differentiation of NPCs co-culture, scale bar 100 μm. (a,f) DAPI (bule); (b,d) Dcx (green). (c,h) Tuj-1 (red); (d,i) merged. (p) The nerve fibers in SCH group were longer than those in the control group. (k–n) Effects of SCH in Primary cortical neuron in vitro neuron with Tuj-1 and Cdc42 positive in SCH group. (o) Tuj-1 positive axon is longer in SCH group than the axon length in the control group. (k2–n2) The Cdc42 is positive in the SCH group neuron. The experiment was performed in three times. N = 10.* p < 0.05, ** p < 0.01, Data are expressed as the mean s. Scale bars: 200 μm.