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Measurements of membrane integrity by SGI/PI staining. Representative contour plots of viable, heat- and C2H5OH-inactivated (A) C. trachomatis EBs/RBs (serovar E) and (B) E. coli stained with SGI/PI are shown in blue. PBS stained with SGI/PI as a reagent control is shown in red. PI-negative (live) bacteria with intact outer membranes are detected in the triangular gate. Threshold settings: SSC 1000.
