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. 2021 Dec 12;10(12):1617. doi: 10.3390/pathogens10121617

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Viability of C. trachomatis measured by flow cytometry over time. (A) Time course of SGI/PI- and CFDA-stained EBs/RBs counts at the same dilution over 0 to 96 h. (B) Measurements of membrane integrity or metabolic activity of EBs/RBs (serovar E, Stock 1CTE) measured by flow cytometry compared to infectivity. The infectivity is calculated from the quotient between IFUs and the DPC measured by epifluorescence microscopy. (C) Long-term measurements of PI-negative and CFDA-positive particle counts during 0 to 44 days (1056 h).