Table 1.

Comparison of VAT’s gene expression data in microarray and RT-qPCR measurements. Values are mRNA level ratio between the different groups (n = 6/8 per group). Microarray experiments identified significant differential expression with p < 0.05 with FDR correction (*) or no FDR correction (a). RT-qPCR validation experiments identified significant differential expression with p < 0.05 (b) or p < 0.1 (c). HFoleic, mice from oleic/control lineage fed with the obesogenic diet; HFepa, mice from EPA/omega 3 lineage fed with the obesogenic diet; Ref, mice from oleic/control lineage fed with the normocaloric diet.

		Microarray			RT-qPCR
Genes	HFoleic vs. Ref	HFepa vs. HFoleic	HFepa vs. Ref	HFoleic vs. Ref	HFepa vs. HFoleic	HFepa vs. Ref
Dnajb1	1.00	1.20 a	1.20a	0.93	1.12 b	1.05
Dock8	4.38 *	0.81	3.53 *	3.56 b	0.82 c	2.92 b
Foxo3	1.10	0.86 a	0.95	0.91	0.85 c	0.77 b
Irs2	0.51 *	1.54 a	0.79	0.49 b	1.38 b	0.68 b
Leptin	2.18 *	0.74 a	1.62	4.97 a	0.46 a	2.30
Mmp13	1.80 *	0.63 a	1.13	4.40 b	0.38 b	1.66
Nupl2	0.96	0.83 a	0.79 *	0.86 c	0.82 b	0.71 b
Plin2	1.94 *	0.76	1.47 a	1.65 b	0.66 b	1.09
Serpine1	0.38 *	2.02 a	0.77	0.37 b	1.94 c	0.72
Stat3	0.78 *	1.13	0.88	0.63 b	0.96	0.60 b
Timp3	0.61 *	1.45 a	0.88	0.59 b	1.18	0.70 b