Table 4.
Percentages and types of cell death observed in the time-lapses of WT and pbr1-8 cells growing in the presence of sublethal (2 µg/mL) or lethal (20 µg/mL) concentrations of the three echinocandin drugs.
| Drug (Concentration) |
Strain | Number of Cells | Cell Death 1 | Cell Lysis 2 | Non-Cell Lysis 3 |
|---|---|---|---|---|---|
| Control (+DMSO) | WT | n = 30 | 0 4 (0) 5 | 0 4 (0) 5 | 0 4 (0) 5 |
| pbr1-8 | n = 33 | 0 (0) | 0 (0) | 0 (0) | |
|
Anidulafungin
(2 µg/mL) |
WT | n = 79 | 89.9 (100) | 65.2 (72.5) | 24.7 (27.5) |
| pbr1-8 | n = 61 | 0 (0) | 0 (0) | 0 (0) | |
|
Anidulafungin
(20 µg/mL) |
WT | n = 60 | 21.7 (100) | 13.3 (61.5) | 8.4 (38.5) |
| pbr1-8 | n = 45 | 8.9 (100) | 2.2 (25) | 6.7 (75) | |
|
Caspofungin
(2 µg/mL) |
WT | n = 96 | 82.3 (100) | 58.3 (70.9) | 24 (29.1) |
| pbr1-8 | n = 85 | 22.4 (100) | 5.9 (26.3) | 16.5 (73.7) | |
|
Caspofungin
(20 µg/mL) |
WT | n = 33 | 27.3 (100) | 12.1 (44.4) | 15.2 (55.6) |
| pbr1-8 | n = 64 | 22.7 (100) | 0 (0) | 22.7 (100) | |
|
Micafungin
(2 µg/mL) |
WT | n = 99 | 80.3 (100) | 13.6 (17) | 69.7 (83) |
| pbr1-8 | n = 36 | 0 (0) | 0 (0) | 0 (0) | |
|
Micafungin
(20 µg/mL) |
WT | n = 61 | 97.5 (100) | 97.5 (100) | 0 (0) |
| pbr1-8 | n = 46 | 0 (0) | 0 (0) | 0 (0) |
1 Cell death is the sum of the percentages of cell lysis and non-cell lysis. 2 Cells shrunk and died with cell breakage and cytoplasm leakage. 3 Cells shrunk and died without cell breakage and cytoplasm leakage. 4 Value is the percentage of dead cells with respect to the total number of visualized cells (n). 5 Value is the percentage of cell lysis or non-cell lysis with respect to the total number of dead cells.