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Effect of heregulin stimulation on HIF-1α expression. Human MCF-7 breast cancer cells were serum starved for 20 h and then exposed to no treatment, 10% FBS (serum), or 25 to 100 ng of heregulin/ml for 6 h, prior to nuclear extract preparation and immunoblot assay using anti-HIF-1α (top) or anti-HIF-1β (bottom) antibodies.
