Skip to main content
. 2021 Dec 18;22(24):13580. doi: 10.3390/ijms222413580

Table 1.

Primers for gene expression using real-time PCR.

Gene Primer Sequence Product Size
β-actin F:5’-TGCGGGACATCAAGGAGAAG-3’ 216
R: 5’-AGTTGAAGGTGGTCTCGTGG-3’
TNF-α F:5’-ATTCAGGGATGTGTGGCCTG-3’ 120
R: 5’-CCAGATGTCCCAGGTTGCAT-3’
IL-6 F: 5’-TGGATAAGCTGCAGTCACAG-3’ 109
R: 5’-ATTATCCGAATGGCCCTCAG-3’
IL-22 F: 5’-TCTCGGTGTAGAGCAAGG-3’ 146
R: 5’-TTCCCAAAGTGCTGGTATT-3’
TLR4 F:5’-CTCCAGCTTTCCAGAACTGC-3’ 192
R: 5’-AGGTTTGTCTCAACGGCAAC-3’
NF-KB F:5’-CTCGCACAAGGAGACATGAA-3’ 147
R:5’-ACTCAGCCGGAAGGCATTAT-3’
IL-17 F:5’-CAGCAAGCTCCAGCTCATCCATC-3’ 92
R:5’-CAGCAGAAGCAGCAGTGACAGG-3’
Claudin-1 F: 5’-GCTGGGTTTCATCCTGGCTTCT-3’ 110
R: 5’-CCTGAGCGGTCACGATGTTGTC-3’
Occludin F: 5’-GTGGTAACTTGGAGGCGTCTTC-3’ 102
R: 5’-CCGTCGTGTAGTCTGTCTCGTA-3’