Figure 5.

Analysis of Al accumulation patterns. (a) Al-treated (25 μM AlCl₃ for 24 h) roots of WT and transgenic soybean lines were stained with hematoxylin, washed, and photographed. Bar = 2 mm. (b) The total Al accumulation in the roots. Soybean seedlings with consistent growth were transferred into a 0.5 μM CaCl₂ hydroponic solution (pH 4.5) containing 0, 10, 25, or 50 μM AlCl₃ for 24 h. The Al of the roots (0–6 cm) was extracted by 2N HCl and determined. The data are the means ± SDs (n = 12). The different letters indicate significant differences at p < 0.05 as determined by Student’s t-test.