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. 2021 Nov 27;14(12):1232. doi: 10.3390/ph14121232

Figure 2.

Figure 2

Figure 2

Antimicrobial peptide analysis. Top: Electrophoresis of bacterial supernatants on Tris–Tricine gels. (A,B) One half of the gel containing bioactive supernatants of the isolates 32YE and 9th, respectively, was challenged with C. michiganensis as a “gel plate assay” and the other half of the gel, with identical samples, was stained with Coomassie Brilliant Blue. (C,D) Gel plate assay of the isolates 33YE or 32LM, respectively, tested against L. monocytogenes and its half-stained gel with identical samples to visualize and recover the protein band(s) for further characterization by MS/MS. (E) MS/MS data from all peptides and protein fragments’ excised bands from all isolates (Supplementary File S6, Table S1). CS: crude supernatant; OP: organic phase; AP: aqueous phase; L: protein standard.