Table 2.

Methods for off-target detection (for further details see [76,77]).

Biased Methods	Unbiased Methods
Amplification of pre-selected off-target sites by PCR analysis followed by Sanger sequencing.	Whole genome sequencing
	Whole exome sequencing.
	ChIP-seq.
	GUIDE-seq.
	Bless.
	IDLVs.
	LAM-HTGTS.
	Digenome-seq.
	CIRCLE-seq.
	SITE-seq.
	GOTI.
	FISH