Figure 1.

Rosemary leaf extract increases myotube cell size. At confluence, myoblasts (M19) were induced to differentiate for three days, and then, rosemary leaf extract was added (RLE) or not (CTRL) at the concentration of 15 μg/mL. Two days later, myotubes were fixed and analyzed by indirect immunofluorescence with an antibody against troponin T. Nuclei were visualized by DAPI staining (blue). (A) Representative images showing troponin T (green) expression. (B) Quantification of troponin T staining (myotube area in μm²). (C) Quantification of the fusion index (percentage). Data are the mean ± SD of five replicates; *** p 0.001. M19, 19-year-old male donor.