Figure 6.

Proteasome activity is inhibited by carnosol. The proteasome chymotrypsin-like activity in protein extracts from myotubes (M19) (A) incubated (CO) or not (CTRL) with carnosol (1 μg/mL) for 48 h or (B) transfected with a control siRNA (siCTRL) or a siRNA against MuRF1 (siMuRF1) was measured by monitoring the hydrolysis of Suc-LLVY-AMC peptide through quantification of AMC fluorescence in the presence or not of bortezomib, a specific proteasome inhibitor; * p < 0.05. Data are the mean ± SD of four replicates. M19, 19-year-old male donor.