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mRNA expression of PCSK9 variants in HEK293 transfected cells. (A) Ct values of PCSK9 mRNA; (B) Ct values of GAPDH mRNA, and (C) relative expression of PCSK9 variants to wt PCSK9. Quantitative real-time PCR (qRT-PCR) was performed in triplicate using iQ SYBR green Supermix (Bio-Rad) on a Real-Time Detection System (Bio-Rad). The mRNA levels were normalized to GAPDH mRNA as a housekeeping gene.
