Table 2.

EC₅₀ values representing the binding affinity of PCSK9 variants to the LDLR determined by solid-phase immunoassay at pH 7.4.

	Mean ± s.d.
wt	120.6 ± 6.6
E32K	50.7 ± 4.8 **
R46L	182.0 ± 32.0 *
L108R	57.7 ± 5.6 **
S127R	50.3 ± 4.7 **
D129G	92.2 ± 5.3 *
D129N	84.0 ± 8.5 *
R194A	204.0 ± 2.3 *
R218S	112.0 ± 2.3 n.s.
D374Y	14.4 ± 0.7 **

* p < 0.025 compared to wt PCSK9; ** p < 0.01 compared to wt PCSK9; n.s. not significant compared to wt.