Figure 4.

Comparative quantitative absolute values of the Trolox equivalent antioxidant capacity of the cell derivative lyophilizate APIs and of the corresponding placebos submitted to the various sample processing methods. Significant intrinsic antioxidant capacities were recorded for both of the API sample groups. The results did not outline statistically significant differences between the whole cell API and the cell lysate API sample groups. The results did not reveal the presence of measurable TEAC values for the placebo sample groups, suggesting that the antioxidant activity observed in the API groups was intrinsic to said APIs. The TEAC values were expressed in µg and corresponded to the Trolox equivalents for the lyophilized APIs (i.e., one API unit, corresponding to 1.5 × 10⁶ cell unit equivalents/vial) reconstituted appropriately with 1.5 mL of aqueous solvent. The results were presented as the mean recorded values from the triplicate experiments, assorted to the corresponding standard deviations as the error bars. API, active pharmaceutical ingredient; TEAC, Trolox equivalent antioxidant capacity.