Figure 2.

The effect of different concentrations of D4-Lnn on induction of apoptosis and necrosis in the cortical cells after 40 min OGD and 24 h reoxygenation: (A) Cytogram demonstrating the viability of cortical cells after OGD/R without pre-incubation with D4-Lnn and after OGD/R with 24 h pre-incubation with different concentrations of D4-Lnn. (B) Comparison of the effect of 10 µg/mL D4-Lnn and Lnn on cell survival after 24 h of OGD/R. Control-cells were not exposed to OGD/R; OGD/R: cells of the cerebral cortex after 24 h OGD/R without pre-incubation with PUFAs; X-axis: the intensity of PI fluorescence; Y-axis: the intensity of Hoechst 33342 fluorescence. Cells were stained with the probes after 24 h the OGD/R. Panels A and B show cells (several hundred) in one experiment. (C) Effects of different D4-Lnn concentrations and 10 µM Lnn on the induction of necrosis and apoptosis after 24 h OGD/R. The percentage of healthy cells and cells with early apoptosis, apoptosis, and necrosis. Statistical significance was assessed using one-way ANOVA followed by the Tukey–Kramer test. Comparison of experimental groups relative to control: n/s—data not significant (p > 0.05), * p < 0.05, ** p < 0.01 and *** p < 0.001. Statistical differences indicated by red asterisks represent comparison of treatment groups relative to OGD/R. Panel C shows the average results obtained from four cell cultures. N (number of animals used for cell cultures preparation) = 4.