Figure 6.

Application of D4-Lnn or Lnn induces the generation of Ca²⁺ signals in astrocytes, but not in neurons of the cerebral cortex: (A) Application of 30 µg/mL D4-Lnn causes the rapid generation of three types of Ca²⁺ signals in astrocytes, and Ca²⁺ responses occur in neurons (single, no more than 0.5% of the total population of neurons) after a lag period. Individual Ca²⁺ responses are presented. (B) Short-term applications (30 s) of increasing concentrations of D4-Lnn to cell cultures of the cerebral cortex. After washing with D4-Lnn, the registration of the [Ca²⁺]_i dynamics was suspended for 10 min. The averaged curves obtained from several tens of cells are presented. (C) Application of 30 µg/mL non-deuterated Lnn causes the rapid generation of biphasic Ca²⁺ signals in astrocytes and does not cause Ca²⁺ responses in neurons. The averaged curves obtained from several tens of cells are presented. Number of parallel coverslips with cell cultures in each analysis = 5. N (number of animals used for cell cultures preparation) = 3.