Table 1.
List of primer sequences used for quantitative real-time PCR.
| Gene | Primer Sequence (5′-3′) | Amplicon Size | Annealing Temperature (°C) |
|---|---|---|---|
| GAPDH | Forward 5′-AGTCCACTGGCGTCTTCA-3′ Reverse 5′-GAGGCATTGCTGATGATCT-3′ |
163 | 58 55 |
| Oct4 | Forward 5′-GGGGGTTCTATTTGGGAAG-3′ Reverse 5′-TTGTCAGCTTCCTCCACCC-3′ |
126 | 57 59 |
| Nanog | Forward 5′-CAGCTACAAACAGGTGAAGACC-3′ Reverse 5′-GGTGGTAGGAAGAGTAAAGGC-3′ |
146 | 62 61 |
| LIF | Forward 5′-GCCCTCTTTATTCTCTATTACACAG-3′ Reverse 5′-ACACGACTATGCGGTACAGC-3′ |
151 | 63 60 |