Table 1.
The effect of caffeine on the relative gene expression (mean ± SEM; n = 6) of pro-inflammatory cytokines receptors in the ovine hypothalamus in basal and lipopolysaccharide-challenge conditions.
| Gene | C/C | LPS/C | C/CAF | LPS/CAF |
|---|---|---|---|---|
|
IL1R1 interleukin 1 receptor 1 |
1 ± 0.1 A | 2.8 ± 0.4 B | 1.3 ± 0.1 A | 2.3 ± 0.3 B |
|
IL1R2 interleukin 1 receptor 2 |
1 ± 0.1 A | 4.5 ± 0.7 B | 1.5 ± 0.1 A | 4.2 ± 0.6 B |
|
IL6R interleukin 6 receptor |
1 ± 0.1 A | 1 ± 0.1 A | 1.1 ± 0.1 A | 1 ± 0.1 A |
|
IL6ST interleukin 6 signal transducer |
1 ± 0.1 A | 1.4 ± 0.1 C | 1.2 ± 0.1 B | 1.3 ± 0.1 B |
|
TNFRSF1A TNF receptor superfamily member 1A |
1 ± 0.1 A | 1.6 ± 0.2 B | 0.8 ± 0.1 A | 1 ± 0.1 A |
|
TNFRSF1B TNF receptor superfamily member 1B |
1 ± 0.1 A | 1.9 ± 0.3 B | 1.1 ± 0.1 A | 1.8 ± 0.1 B |
C/C—double treated with saline (0.9% NaCl iv.), LPS/C—treated with lipopolysaccharide (LPS, 400 ng/kg of bm., iv.) followed by saline, C/CAF –treated with saline followed by caffeine (CAF, 30 mg/kg of body mass (bm.), iv.), LPS/CAF—treated with LPS followed by CAF. Gene expression data were normalized to the average relative level of this mRNA expression in the control sheep (C/C), which was set to 1.0. Different letters indicate significant differences at p < 0.05, according to one-way ANOVA followed by Fisher’s post hoc test comparing groups with each other.