FIGURE 1.

Sertaconazole inhibits NSCLC cell growth in vitro and in vivo. (A) The chemical structure of sertaconazole (STZ). (B) Cell growth of human NSCLC cell lines and the bronchial epithelial cell line 16HBE treated with sertaconazole at indicated concentrations. (C, D) A549 and H460 cells were treated with sertaconazole for 48 h. Cell proliferation was determined by EdU incorporation (C) and colony formation (D). (E) Cells were incubated with sertaconazole for 48 h, and cytotoxicity was detected by analyzing the release of LDH. (F–H) A549 cells were subcutaneously inoculated into nude mice. Mice were intraperitoneally injected with vehicle or sertaconazole. Image (F), volume (H), and weight (G) of tumor xenografts were measured. (I, J) Immunohistochemical staining of Ki67 in tumor xenografts. Scale bar, 50 μm. All experiments were repeated at least three times. Statistic method: 2‐tailed Student's t‐test. Data are means with SD. **p < 0.01, ***p < 0.001