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. 2021 Dec 16;2(4):821–837. doi: 10.1002/mco2.102

FIGURE 3.

FIGURE 3

Sertaconazole induces autophagy in NSCLC cells. (A) Immunoblotting of ATG7, Beclin 1, p62, ATG5, and LC3 turnover in A549 and H460 cells treated with sertaconazole for 24 h. (B) Immunoblotting of LC3 turnover in A549 and H460 cells treated with the indicated times of sertaconazole. (C) Immunohistochemical staining of LC3 in tumor xenografts. Scale bar, 50 μm. Statistic method: 2‐tailed Student's t‐test. (D, E) Immunofluorescent analysis of endogenous LC3 puncta in cells treated with or without sertaconazole for 24 h. Scale bar, 10 μm. Statistic method: 2‐tailed Student's t‐test. (F) The interaction between Beclin 1 and Bcl‐2 in response to sertaconazole treatment was analyzed by reciprocal coimmunoprecipitation assay. (G) A549 and H460 cells were treated with sertaconazole in the absence or presence of 3‐MA for 24 h. The LC3 turnover was detected by immunoblotting. (H) Cells were transfected with siAtg5 or siScramble, followed by treatment with sertaconazole for 24 h. The LC3 turnover was detected by immunoblotting. All experiments were repeated at least three times. Data are means with SD. ***p < 0.001