Table 1.
Gene | Primer Orientation | Nucleotide Sequence |
---|---|---|
b-actin | forward | 5′-AGAGCTATGAGCTGCCTGACG-3′ |
reverse | 5′-CCGCAAGATTCCATACCCA-3′ | |
Oxidative stress pathway genes |
||
sod1 | forward | 5′-GGCCAACCGATAGTGTTAGA-3′ |
reverse | 5′-CCAGCGTTGCCAGTTTTTAG-3′ | |
cat | forward | 5′-AGGGCAACTGGGATCTTACA-3′ |
reverse | 5′-TTTATGGGACCAGACCTTGG-3′ | |
gstp2 | forward | 5′-CACAGACCTCGCTTTTCACAC-3′ |
reverse | 5′-GAGAGAAGCCTCACAGTCGT-3′ | |
Nrf2 | forward | 5′-TCGGGTTTGTCCCTAGATG-3′ |
reverse | 5′-AGGTTTGGAGTGTCCGCTA-3′ | |
Apoptosis pathway genes | ||
casp-3 | forward | 5′-CCGCTGCCCATCACTA-3′ |
reverse | 5′-ATCCTTTCACGACCATCT-3′ | |
Bax | forward | 5′-GGCTATTTCAACCAGGGTTCC-3′ |
reverse | 5′-TGCGAATCACCAATGCTGT-3′ | |
bcl-2 | forward | 5′-TCACTCGTTCAGACCCTCAT-3′ |
reverse | 5′-ACGCTTTCCACGCACAT-3′ | |
casp-9 | forward | 5′-AAATACATAGCAAGGCAACC-3′ |
reverse | 5′-CACAGGGAATCAAGAAAGG-3′ |