Figure 3.

Autophagy inhibition regulates APG-induced cell death. (A–F) After AGS and SNU-638 cells were transfected with LC3B and ATG5 siRNAs, cell viability, LDH production, and Western blot analyses were performed with/without APG (50 μM, 24 h) treatment. Cell viability and LDH activity were determined using WST-1 and LDH assays, respectively; * p < 0.05, n.s; no significant. Western blotting was performed to identify the autophagy-related genes ATG5 and LC3B in APG-treated ATG5 or LC3B knockdown cells. β-actin was used as a protein loading control.