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Particle characterizations of R848 NEs. (A) Particle sizes of R848 NEs with lipid-to-R848 weight ratio of 20:1, 15:1, 10:1, 5:1, and 2:1. (B) SEC chromatogram of R848 NE using a Sepharose CL-4B gel column. Absorbance at 320 nm was measured for the presence of R848. (C) Particle stability of empty NE and R848 NE stored at 4 °C, up to 3 weeks.
