Figure 3.
Transfection efficiency of the LNPssPalm and other transfection methods. (a) Comparison with the general transfection procedures. The transfection activity of the LNPssPalm was compared to the electroporation (EP) or a commercially available lipofection reagent (lipofectamine messengerMAX; LFN). The luminescence from the cultured cells was measured for 2 min in the incubator-type luminometer. Cumulative light unit for 2 min was plotted against time, transfected with mRNA. The mean of the independent experiments is shown (n = 3). (b) Cumulative luciferase activity. The transfection activity was accumulated for 48 h. Data were represented as mean ± SD (n = 3). Statistical analysis was performed by One-way ANOVA followed by Tukey–Kramer test, **; p < 0.01 against LFN.