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. 2021 Dec 6;13(12):2097. doi: 10.3390/pharmaceutics13122097

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Integrated stress responses induced by the transfection. The phosphorylation level of the eIF2α was compared. Jurkat cells were fixed and permeabilized after the transfection. The intracellular amount of phosphorylated eIF2α was evaluated by an anti-phosphorylated eIF2α antibody. The phosphorylation level of the eIF2α of LNP_ssPalm and electroporation (EP) was compared to the non-treated cells (NT). Data were represented as mean ±SD (n = 3). Statistical analyses were performed by One-way ANOVA followed by Tukey–Kramer test, *; p < 0.05 against non-treated group **; p < 0.01 against non-treated group.