Figure 6.

Uptake mechanism of the LNP_ssPalm. (a) The effects of temperature. The Jurkat cells were incubated with the LNP_ssPalm at 37 °C or 4 °C. The cellular uptake was evaluated by flow-cytometry. Data are represented as the mean ± SD (n = 3). (b) pharmacological inhibition of the endocytosis. The Jurkat cells were incubated with LNP_ssPalm in the presence of pharmacological inhibitors of endocytosis pathways. As inhibitors, 30 µM Pitstop^® 2 (clathrin inhibitor), 20 µM Dynole^® 34-2 (dynamin inhibitor), 100 µM Genistein (tyrosine kinase inhibitor), and 10 µM Cytochalasin D (actin inhibitor) were used. Data were represented as mean ±SD (n = 3). Statistical analyses were performed by Scheffe’s F multicomparison test (n = 3), **; p < 0.01.