Table 1.
Particle properties of the LNPs in Figure 2.
| Helper | PEG Content | Size (nm) 1 | PdI 1 | Zeta Potential (mV)1 | mRNA Encapsulation (%) 2 |
|---|---|---|---|---|---|
| DOPC | 0.375% | 110.4 ± 8.7 | 0.15 ± 0.02 | −7.16 ± 0.59 | 73.9 ± 1.6 |
| 0.75% | 66.5 ± 5.4 | 0.10 ± 0.01 | −4.47 ± 0.21 | 84.0 ± 1.0 | |
| 1.5% | 46.1 ± 4.7 | 0.08 ± 0.03 | −3.15 ± 0.21 | 85.4 ± 1.1 | |
| 3% | 36.1 ± 5.1 | 0.12 ± 0.06 | −2.09 ± 0.44 | 84.7 ± 1.2 | |
| POPE | 0.375% | 106.0 ± 3.6 | 0.15 ± 0.00 | −8.3 ± 0.67 | 79.0 ± 0.7 |
| 0.75% | 63.2 ± 4.3 | 0.12 ± 0.01 | −5.9 ± 0.49 | 85.8 ± 2.1 | |
| 1.5% | 45.5 ± 0.6 | 0.08 ± 0.00 | −4.7 ± 0.53 | 85.6 ± 2.8 | |
| 3% | 38.9 ± 2.8 | 0.11 ± 0.01 | −2.7 ± 0.57 | 83.9 ± 1.3 |
1 Size, PdI, and Zeta potential were evaluated by dynamic light scattering. 2 mRNA encapsulation was evaluated by RibogreenTM assay.