Figure 1.

(A) Growth curve of Lactobacillus iners. L. iners ATCC 55195 was incubated for 52 h in brain heart infusion (BHI) broth, and bacterial growth was determined at selected time points. Values represent mean ± SEM of colony-forming units (CFU)/mL from three independent experiments performed in duplicate. (B) Biofilm formation by Candida albicans clinical isolates in vitro. The isolates were grown for 24, 48, and 72 h. Biofilm biomass was evaluated using the crystal violet staining method, and values represent mean ± SEM of absorbance at OD₅₉₅. Data are from at least three independent experiments with n = 6. (C) Biofilm formation by C. albicans clinical isolates in vitro in RPMI 1640 + BHI broth. Biofilms were grown for 24 h using 1:1 RPMI 1640 and BHI broth. Values represent mean ± SEM of absorbance at OD₅₉₅ from at least three independent experiments with n = 6.