Table 3.
Primers used for semi- and quantitative RT-PCR analysis.
| Primer | Forward (5′-3′) | Reverse (5′-3′) | Annealing Temperature (°C) |
|---|---|---|---|
| OPN | GCCGACCAAGGAAAACTCACT | GGCACAGGTGATGCCTAGGA | 64.7 |
| OCN | AGCAAAGGTGCAGCCTTTGT | GCGCCTGGGTCTCTTCACT | 63.2 |
| ADIPO2 | GGTGGTGGAATGCGTCATG | CAACGTCCCTTGGCTTATGC | 64.1 |
| ALP | GGAACTCCTGACCCTTGACC | TCCTGTTCAGCTCGTACTGC | 65.2 |
| PPARγ | CCGAGAAGGAGAAGCTGTTG | TCGGATATGAGAACCCCATC | 60.8 |
| RPL13a | AAGTACCAGGCAGT GACAG | CCTGTTTCCGTAGCCTCATG | 58 |
| SOX9 | GACTTCCGCGACGTGGAC | GTTGGGCGGCAGGTACTG | 57.5 |
| COL2A | AGACTGGCGAGACTTGCGTCTA | ATCTGGACGTTGGCAGTGTTG | 57.3 |