Figure 5.

Immunofluorescence microscopy of P. salmonis-infected salmon cell lines and zebrafish primary cell cultures. (A) P. salmonis-infected and mock-infected (control) salmon cell lines SHK-1 and ASK were fixated before fluorescence staining after 6 and 12 dpi. (B) P. salmonis-infected and mock-infected (control) zebrafish primary cultures (ZKPCC) were fixated after 1 and 5 dpi. Both panels show representative images of zebrafish cells with different cell types. For the three cultures, cellular actin was stained with phalloidin in green (Alexa Fluor 488^®), and the nucleus in blue with DAPI. Polyclonal antibodies against P. salmonis and a secondary antibody coupled with Alexa Fluor 594^® marked the bacteria in red. Bar represents 50 µm. Images obtained at each channel are provided in Figure S3.