Figure 6.

Host and pathogen biomarker’s gene expression during P. salmonis infection. Heat map showing relative transcript abundance of immune-related genes in P. salmonis-infected cultures and virulence factor genes in P. salmonis at early- and late-stage infections. (A) Expression level of host biomarkers in SHK-1 and ASK cell lines at 6- and 12-dpi. (B) Expression level of host biomarkers in ZKPCC at 1- and 5-dpi. In (A,B), the gene expression of cell cultures infected with P. salmonis is presented as relative to uninfected cell cultures. (C) The gene expression of P. salmonis biomarkers during infection inside cell cultures is presented as relative to P. salmonis growing in Austral-SRS medium. The average of ΔΔCt values for three independent replicates is shown in all heat maps, and non-significant values (between +2- and −2-fold changes) are shown in white.