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. 2021 Dec 18;9(12):2618. doi: 10.3390/microorganisms9122618

Figure 1.

Figure 1

Progress curve of V. cholerae culturability and viability during the course of VBNC induction.(a) Culturable cell count was determined for V. cholerae AN59 during the induction of VBNC state at 4 °C (●) in ASW. Recovery was induced on days 56, 63, 70 and 77 after entry into VBNC state by temperature upshift from 4 °C to 37 °C (●). Each bar represents the mean ± SE of three independent experiments. (b) The relative mRNA expression of transcripts of molecular chaperones (■) dnaK and (●) GroEL were analyzed during the course of VBNC induction as an indicator of cell viability.