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. 2021 Dec 18;9(12):2618. doi: 10.3390/microorganisms9122618

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of protease on recovery of V. cholerae from the VBNC state is a general trait. Recovery was induced in VBNC cells at 37 °C in spent ASW media (SM) and heat-treated spent ASW media (∆SM) without or with proteinase K, subtilisin, trypsin and BSA. The recovered cell count was determined after 32 h. Asterisk (*) represents statistically significant difference in growth of VBNC cells with and without proteases in spent ASW media (p < 0.05). # represents non-significant difference in growth of VBNC cells with and without proteases in heat-treated spent ASW media.