. 2021 Dec 5;14(12):1262. doi: 10.3390/ph14121262

Table 3.

Anti-QS and anti-virulence properties of RhlR-modulating QSIs.

QSIs	Anti-QS Properties		Anti-Virulence Properties			Ref.
	Target	% Inhibition/Activation (IC₅₀/EC₅₀)	Anti-Biofilm Activity	Anti-Rhamnolipid Activity	Anti-Pyocyanin Activity
	Target	% Inhibition/Activation (IC₅₀/EC₅₀)	% Inhibition	% Inhibition	IC₅₀
24	RhlR (agonist)	43% at 1 mM ^(a)	≈40% at 100 µM ^(e)	ND	8 µM ^(g)	[100]
24	RhlR (agonist)	80% at 20 µM ^(b)	≈40% at 100 µM ^(e)	ND	8 µM ^(g)	[100]
25	RhlR (agonist)	4.5 µM ^(c)	ND	ND	ND	[101]
26	RhlR (agonist)	7.2 µM ^(c)	ND	ND	ND	[101]
27	RhlR (antagonist)	20% at 10 µM ^(d)	15% at 10 µM ^(f)	≈40% at 10 µM ^(h)	NA ^(h)	[105]

NA = Not active, ND = Not Determined; Anti-QS evaluations carried out using reporter gene assays on/with (a) E. coli (rhlA-gfp)/competition against odDHL (100 nM); (b) E. coli (rhlA–gfp); (c) P. aeruginosa (PAO-JP2, prhlILVAGFP); (d) P. aeruginosa (PAO1, rhlA-lacZ); Anti-virulence evaluations carried out on/by (e) static biofilm assay with P. aeruginosa (PA14)/confocal laser scanning microscopy; (f) static biofilm assay with P. aeruginosa (PAO1)/CV dyeing and OD measurement; (g) P. aeruginosa (PA14)/OD measurement; (h) P. aeruginosa (PAO1)/OD measurement.