. 2021 Dec 5;14(12):1262. doi: 10.3390/ph14121262

Table 8.

Anti-QS and anti-virulence of multi-target QSIs.

QSIs	Anti-QS Properties		Anti-Virulence Properties		Ref.
	Target	IC₅₀	Anti-Biofilm Activity	Anti-Pyocyanin Activity
	Target	IC₅₀	IC₅₀	IC₅₀
57 (M59)	PqsBC	13.4 µM ^(a)	ND	ND	[132]
57 (M59)	PqsR	ND	ND	ND	[132]
58	PqsR	15 µM ^(b)	100 µM ^(d)	86 µM ^(c)	[133,134]
58	PqsD	21 µM ^(c)	100 µM ^(d)	86 µM ^(c)	[133,134]

ND = Not Determined; Anti-QS evaluations carried out using reporter gene assays on/with (a) PqsBC enzyme in presence of 2-ABA and octanoyl-CoA; (b) E. coli (DH5α, pEAL08-2); (c) PqsD enzyme in presence of anthranilyl-CoA (5 μM) and β-ketodecanoic acid (70 μM); Anti-virulence evaluations carried out on/by (d) static biofilm assay with P. aeruginosa (PA14)/CV dyeing and OD measurement; (e) P. aeruginosa (PA14)/OD measurement.