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. 2021 Nov 28;13(12):2380. doi: 10.3390/v13122380

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of binding activities of 9 mAbs. (A,B) Detection of gp42 on the surface of full-length gp42 transfected 293T cells using (A) fluorescence activated cell sorting (FACS) and (B) immunofluorescence (IF) assays. Antibodies were labeled with (A) GAM-BV421 and (B) GAM-Alexa-Fluor 488. Anti-gp350 murine antibody 72A1 was used as negative control. (C) In total, 9 mAbs were tested by Western blot for reactivity against denatured GST-fused gp42-44-61aa, GST-fused gp42-67-81aa and gp42 proteins.