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. 2021 Nov 28;13(12):2380. doi: 10.3390/v13122380

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Generation and characterization of chimeric VLPs. (A) Schematic diagram illustrating the design of chimeric VLPs carrying the peptides from gp42 N-terminal region. gp42-44-61aa is labelled in cyan and gp42-67-81aa is labelled in green. (B) SDS-PAGE and Western blot analysis of fusion proteins. Anti-HBc149 mAb 1B11 was used for particle detection. (C) The size and morphology analysis evaluated by transmission electron microscope (TEM) and high-performance liquid chromatography (HPSEC). (D) Reactivities of chimeric VLPs with anti-gp42 immune serum and anti-HBc149 mAb 1B11 using ELISA assay.