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. 2021 Dec 20;22(24):13641. doi: 10.3390/ijms222413641

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescent pictures of the nucleus pulposus (NP) and in the annulus fibrosus (AF). The sections were stained with DAPI (blue) and anti-YAP (green) and are shown individually as well as merged. (a) NP cells at day 0, (b) NP cells after static load, (c) NP cells after low-stress load, (d) NP cells after intermediate-stress load, (e) NP cells after high-stress load, (f) AF cells at day 0, (g) AF cells after static load, (h) AF cells after low-stress load, (i) AF cells after intermediate-stress load, (j) AF cells after high-stress load. Scale bar = 100 µm.