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Negative-ion MALDI MS/MS spectrum of precursor ion at m/z 1343.6, a representative ion peak of the cluster ascribed to tri-acylated lipid A species from E. vietnamensis KMM 6221T. The assignment of fragments is reported. The proposed structure for the lipid A species is sketched in the inset carrying two negative charges for information purposes only in order to underline that both sites of the molecule (i.e., the phosphate and the GalA) may hold the negative charge.
