Figure 7.

Stimulation of HEK Blue^TM hTLR4 cells. SEAP levels (OD) (a) and IL-8 release (pg/mL) (b) upon stimulation with LPS of E. pacifica KMM 6172^T (1, 10, 100 ng/mL) or E. vietnamensis KMM 6221^T LPS (1, 10, and 100 ng/mL); S. typhimurium SH 2201 LPS was used as positive control. Significant differences between E. pacifica KMM 6172^T LPS or E. vietnamensis KMM 6221^T LPS and S. typhimurium LPS values are indicated. ** p < 0.01, *** p < 0.001 by the Student t-test. NT, not treated cells. Data are expressed as mean ± SD of three independent experiments in triplicate.