Figure 3.

LOBCE stimulates PMN adhesion to matrix proteins. PMNs (5 × 10⁴ cells per well) were incubated with CMFDA (10 μM) for 30 min. Then, PMNs were washed, incubated in the presence or absence of LOBCE (1–10 μg/mL), and seeded in a 96-well black plate which was previously coated with (A) collagen IV, (B) fibrinogen, or (C) fibronectin for 30 min at 37 °C in a 5% CO₂ air atmosphere. After this time, the plate was washed, and the adherent PMNs were measured through CMFDA fluorescence at excitation and emission wavelengths of 492 and 517 nm, respectively. * p < 0.05 compared to the control group, ** p < 0.01, compared to the control group (n = 3).