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The PCR cycle steps, durations and temperatures of the runs.
| PCR Cycle Step | Temperature (°C) | Time (min:sec) |
|---|---|---|
| Initial denaturation | 94 | 47:00 |
| Denaturation | 94 | 00:47 |
| Annealing | 52 | 00:60 |
| Elongation | 72 | 01:30 |
| Final elongation | 72 | 10:00 |
°C, Celsius. PCR, polymerase chain reaction; min:sec, minutes:seconds.
