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. 2021 Dec 15;10(12):1628. doi: 10.3390/pathogens10121628

Figure 2.

Figure 2

Role of MbovP0145 in IL-8 expression in EBL cells. (A) The expression of MbovP0145 in the M. bovis HB0801, mutant strain T6.93, and complemented strain CT6.93, respectively. Total cell lysates of each strain were separated on 10% SDS-PAGE gel, transferred to a polyvinylidene fluoride membrane, and treated with anti-MbovP0145 and anti-MbovP579 antibodies. (B) M. bovis promotes IL-8 mRNA expression in EBL cells. EBL cells were infected with M. bovis HB0801, T6.93, or CT6.93. Cells were collected at different time points, and qRT-PCR was performed to quantify the IL-8 expression. PBS was used as a negative control. (C,D) EBL was stimulated by rMbovP0145 at different concentrations and times. The relative expression of IL-8 was analyzed as above. Data are the means of three independent assays. Standard deviations are indicated by error bars. p-values are indicated by asterisks (*** p < 0.001, ** p < 0.01, * p < 0.05, and ns = p > 0.05).