Figure 7.

The mutant strain of MbovP0145 impaired the ability of neutrophil recruitment. Neutrophils were assayed for chemotaxis using a conditioned medium collected after the exposure of EBL cells to M. bovis (HB0801), the Mbov_0145 mutant strain (T6.93), and the complementary strain (CT6.93). DMEM or DMEM from uninfected EBL cells served as the negative control. After being cocultured 8 h, the neutrophils that migrated to the lower chamber were collected and counted. Data are the means of three independent assays. Standard deviations are indicated by error bars. p-values are indicated by asterisks (*** p < 0.001, * p < 0.05, and ns = p > 0.05).