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. 2021 Dec 12;14(24):7648. doi: 10.3390/ma14247648

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence experiments on ARPCs showing a different pattern of growth on DHT and DHT/EDC films compared to SIS patch. Stem cells were stained for β-Actin (green) and CD133 (red); nuclei were counterstained with DAPI (blue). Original Magnification: 63×. Panels (A,B) represent ARPCs cultured on a SIS patch and stained for β-Actin (green, (A)) and CD133 (red, (B)). Panels (C,D) represent ARPCs cultured on DHT film and stained for β-Actin (green, (C)) and CD133 (red, (D)). Panels (E,F) represent ARPCs cultured on DHT/EDC film and stained for β-Actin (green, (C)) and CD133 (red, (D)). Panels (G–I) represent spheroids generated from DHT/EDC films and stained for β-Actin (green, (G)) and CD133 (red, (H)). Panel (I) represents the overlay between the β-Actin and CD133 staining.