Figure 6.

Relative expression of genes involved in the biosynthesis (A), degradation (B), and recycling (C) of AsA in the flavedo of fruit of Valencia Late orange (Citrus sinensis) during fruit development and maturation. Fruits were harvested from early development in June (Jn) to overmature fruit in June of the next year (indicated as Jn*). The genes analyzed were GMP, GME, GGP, GPP, GalDH, and GalLDH of the L-galactose biosynthesis pathway; GalUR5, GalUR8, GalUR10, GalUR12, GalUR14, and GalUR17 of the D-galacturonic bio-synthesis pathway; MIOX, of the myo-inositol pathway; AO, APX1, APX2, APX3, and APX4 in-volved in AsA degradation; and MDHAR1, MDHAR2, MDHAR3, DHAR1, and DHAR2, involved in AsA recycling. An expression value of 1 was arbitrarily assigned to the values obtained in the flavedo of fruit in June. Results are the mean ± S.E of at least three replicates.