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. 2021 Dec 18;22(24):13592. doi: 10.3390/ijms222413592

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of the cell entry of SARS-CoV-2 pseudotyped particles. The 293 T cells transiently expressing human ACE2 and TMPRSS2a were pre-treated with the selected compounds at the concentrations indicated for 3 h or with 20 μg/mL of the human anti-ACE2 antibody (positive control) before they were inoculated with SARS-CoV-2-specific spike protein pseudotyped lentivirus particles (spike virus) or particles without a viral envelope (Δ spike). A group of cells inoculated with spike virus or Δ spike was left untreated. At 48 h postinoculation, pseudotype virus entry was analyzed by luminescence readout (normalization against untreated spike virus entry). Data represent the mean ± SEM from three independent experiments carried out in technical triplicates. Unpaired two-tailed t-test analysis was used to calculate statistical significance. One biological experiment of Otamixaban was excluded due to technical error. Concentrations are displayed in logarithmic scale and molar concentrations. The logarithmic scale is used for better visibility of the wide range of concentrations that are used in these assays.