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MTS assay of cell viability indicated by mitochondrial activity. HEK293 cells co-transfected with hACE2 and hTMPRSS2a were treated with 1 and 10 µM of candidate for 40–44 h. Assays were performed according to the manufacturer’s instructions. The mitochondrial activity in untreated cells was assigned a value of 100. Experiments were performed in one to three biological replicates and technical triplicates. Bars indicate mean ± standard deviation.
